Composite

Part:BBa_K1084123:Design

Designed by: Matsumura Naoya   Group: iGEM13_HokkaidoU_Japan   (2013-09-06)

pTet SD6 LacZα dT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is constructed to measure translation efficiency of SD6 (BBa_K1084103). We used LacZα (BBa_I732006) as reporter gene and performed β-Galactosidase assay.


Source

Based on Vimberg et al. (2007), we construnted this part from synthetic oligos.

References

V. Vimberg, A. Tats, M. Remm T. Tenson, Translation initiation region sequence preferences in Escherichia coli (2007) BMC Molecular Biology