Regulatory

Part:BBa_K108025:Design

Designed by: Qi Liu   Group: iGEM08_Tsinghua   (2008-10-23)

Pu


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 163
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.


Source

Pu was cloned from pWW0 of Pseudomonas putida (reference 1 and 2).

References

1. Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulators. Annual Review of Microbiology. 1997, 51:341-373.

2. NCBI.