Regulatory

Part:BBa_K108014:Design

Designed by: Qi Liu   Group: iGEM08_Tsinghua   (2008-10-23)

PR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 136
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR using primers with biobrick prefix and surfix, cloned into pSB1AC3, and then eliminate Pst1 using site-directed mutation.


Source

PR was cloned from Alcaligenes eutrophus H16 genome (reference 1 and 2).

References

1. Regulation of phasin expression and polyhydroxyalkanoate (PHA) granule formation in Ralstonia eutropha H16. Microbiology. 2002, 148:2413–2426.

2. NCBI.