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Part:BBa_K1075047:Design

Designed by: Marc Schulte   Group: iGEM13_Bonn   (2013-09-23)

pDawn-RBS32-ccdB-TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2171
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 63
    Illegal NgoMIV site found at 195
    Illegal NgoMIV site found at 289
    Illegal NgoMIV site found at 582
    Illegal NgoMIV site found at 1076
    Illegal NgoMIV site found at 1094
    Illegal NgoMIV site found at 1184
    Illegal AgeI site found at 414
    Illegal AgeI site found at 1542
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1643
    Illegal BsaI site found at 3659
    Illegal BsaI.rc site found at 525


Design Notes

This part is a combination of the light-inducible BBa_K1075044 and the toxin ccdB (BBa_K1075029).


Source

The origin of the toxin CcdB (BBa_K1075029) is the bacteria Escherichia coli.

References

[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2958.2001.02492.x/abstract;jsessionid=6CE0056B73608A461539FF28D894B906.f03t01]Afif H, Allali N, Couturier M, Van Melderen L.(2001)The ratio between CcdA and CcdB modulates the transcriptional repression of the ccd poison-antidote system.

[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1635281/]Andrew B. Smith and Anthony Maxwell(2006)A strand-passage conformation of DNA gyrase is required to allow the bacterial toxin, CcdB, to access its binding site.

[http://www.ncbi.nlm.nih.gov/pubmed/?term=A+Common+Origin+for+the+Bacterial+Toxin-Antitoxin+Systems+parD+and+ccd]Smith AB, López-Villarejo J, Diago-Navarro E, Mitchenall LA, Barendregt A, Heck AJ, Lemonnier M, Maxwell A, Díaz-Orejas R.(2012)A common origin for the bacterial toxin-antitoxin systems parD and ccd, suggested by analyses of toxin/target and toxin/antitoxin interactions.

[http://www.sciencedirect.com/science/article/pii/S0022283612000113] Ohlendorf et al. (2009) From Dusk till Dawn One-Plasmid Systems for Light-Regulated Gene Expression.