Regulatory

Part:BBa_K1075044:Design

Designed by: Max Schelski   Group: iGEM13_Bonn   (2013-09-23)

Promoter(const.)-RBS34-YF1-FixJ-FixK-LambdaC-pC (pDawn)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2171
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 63
    Illegal NgoMIV site found at 195
    Illegal NgoMIV site found at 289
    Illegal NgoMIV site found at 582
    Illegal NgoMIV site found at 1076
    Illegal NgoMIV site found at 1094
    Illegal NgoMIV site found at 1184
    Illegal AgeI site found at 414
    Illegal AgeI site found at 1542
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1643
    Illegal BsaI.rc site found at 525


Design Notes

pDawn was designed by Moglich et al. [1]


Source

PCR amplification from pDawn, triple mutagenesis to remove EcoRI, PstI and XbaI

References

[http://www.sciencedirect.com/science/article/pii/S0022283612000113] Ohlendorf et al. (2009) From Dusk till Dawn One-Plasmid Systems for Light-Regulated Gene Expression.