Composite

Part:BBa_K1073030:Experience

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-17)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1073030

By adding autoinducer N-buturyl-HSL to the media the expression of beta lactamase can be induced. The iGEM Team Braunschweig 2013 used this device in combination with BBa_K1073033 for the construction of BBa_K1073034.

User Reviews

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BBa_K1073030 iGEM Team Braunschweig 2013

This device was used for the construction of BBa_K1073034. With this construct in a high copy plasmid (pSB1C3) we were able to induce expression of beta lactamase in E. coli JM109 when N-3-oxododecanoyl-HSL was added to the media and thus maintain cells growth in complex medium containing ampicillin. For detailed experience see BBa_K1073034.

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