Composite

Part:BBa_K1072017:Design

Designed by: Junqi kuang   Group: iGEM13_SCUT   (2013-09-21)


pGAL1 + Flag + odr-10 + GFP + ADH1Te + pFUS1 + BFP + ADH1Te


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2258


Design Notes

-


Source

olfactory receptor Odr-10 of C.elegans and kit plates

References

1. Olesnicky NS, Brown AJ, Dowell SJ, Casselton LA.A constitutively active G-protein-coupled receptor causes mating self-compatibility in the mushroom Coprinus.EMBO J. 1999 May 17;18(10):2756-63.

2. Sengupta P, Chou JH, Bargmann CI.odr-10 encodes a seven transmembrane domain olfactory receptor required for responses to the odorant diacetyl.Cell. 1996 Mar 22;84(6):899-909.

3. Dowell SJ, Brown AJ.Yeast assays for G-protein-coupled receptors.Receptors Channels. 2002;8(5-6):343-52.