Composite

Part:BBa_K1072006:Design

Designed by: Ruichen, Yichong wang and Junjie zhang   Group: iGEM13_SCUT   (2013-09-07)


Lux pR+RBS+α-ALS+2TM


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

Promotor, RBS and terminator get from registry part and α-ALS gene originate from Lactococcus lactis, respectively.

References

1. Chuang LF, Collins EB. Biosynthesis of diacetyl in bacteria and yeast．Journal of Bacteriology．1968,95(6)：2083-2089．

2. Snoep JL，Teixeira De Mattos MJ，Starrenburg MJ，et a1.Isolation, characterization, and physiological role of the pyruvate dehydrogenase complex and alpha-acetolactate synthase of Lactococcus lactis subsp. lactis bv. diacetylactis.．Joumal of Bacteriology，1992,174 (14)：4838．41.