Composite

Part:BBa_K1072000:Design

Designed by: Yichong wang, Rui chen, Xihao liao and Junjie zhang   Group: iGEM13_SCUT   (2013-08-31)

Lux pL controlled LuxR + lux pR autoinducing LuxI (lva tag) + lux pR controlled GFP.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1821
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101
    Illegal BsaI.rc site found at 1970
    Illegal BsaI.rc site found at 2697


Design Notes

Constructing device compositing oscillator, coupling, reporter and productor.This part include the oscillator and reporter.

Source

Get from kit plate

References

1. Dong YH, Zhang LH.Quorum sensing and quorum-quenching enzymes.J Microbiol. 2005 Feb;43 Spec No:101-9.

2. Danino T, Mondragón-Palomino O, Tsimring L, Hasty J. A synchronized quorum of genetic clocks. Nature. 2010 Jan 21;463(7279):326-30.

3. Prindle A, Samayoa P, Razinkov I, Danino T, Tsimring LS, Hasty J.a sensing array of radically coupled genetic biopixels. Nature. 2011 Dec 18;481(7379):39-44.