Coding

Part:BBa_K1067003:Design

Designed by: Kristian Davidsen and Helen Cook   Group: iGEM13_DTU-Denmark   (2013-09-19)

Ammonium monooxygenase from Nitrosomonas europaea


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 263
    Illegal BamHI site found at 306
    Illegal BamHI site found at 2510
    Illegal BamHI site found at 2822
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2028
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick includes the enzyme ammonia monooxygenase (AMO), which performs first step of the nitrification pathway and converts ammonia into hydroxylamine. The gene is taken from Nitrosomonas europaea. Use this part on its own to produce hydroxylamine, or use with BBa_K1067004 (hydroxylamine oxidoreductase) and BBa_K1067002 (cytochromes associated with hydroxylamine oxidoreductase) to complete the nitrification pathway. BBa_K1067005 includes all three components on one plasmid.


Genes taken from Nitrosomonas europaea ATCC 19718 by colony PCR.

Source

Link to AMO on [http://www.uniprot.org/uniprot/Q04507 uniprot].

References