Plasmid_Backbone

Part:BBa_K106693:Design

Designed by: Andrew Horwitz   Group: iGEM08_UCSF   (2008-10-28)


AarI A!D acceptor vector (pRS315, Cyc1P, Adh1t)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2218
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2218
    Illegal NotI site found at 22
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2218
    Illegal BamHI site found at 5
    Illegal XhoI site found at 924
    Illegal XhoI site found at 7641
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2218
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2218
    Illegal NgoMIV site found at 535
    Illegal NgoMIV site found at 6360
    Illegal AgeI site found at 1831
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 4768
    Illegal BsaI site found at 7010
    Illegal SapI.rc site found at 3746
    Illegal SapI.rc site found at 5850


Design Notes

Note that this vector will NOT accept standard AarI "A" parts.


Source

Modified pRS315 plasmid.

References