Device
Part:BBa_K1065105:Design
Designed by: Gabriele Girelli Group: iGEM13_UNITN-Trento (2013-08-14)
lac_promoter + RBS + SAM synthetase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 324
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 904
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene sequence was extracted using the following primers:
>Primer Forward: prefix + RBS + spacer + ATG...
GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT
>Primer Reverse: sequence_TAATAA_suffix
GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT
SAM synthetase was inserted into BBa_R0010 through standard assembly.
Source
SAM synthetase gene sequence extracted by PCR from E. coli MG1655 strain genome.
References
-
Ethylene biosynthesis and its regulation in higher plants
Yang, S. F.; Hoffman N. E. Ann. Rev. Plant Physiol. 1965, 35, 155-89. - MetaCyc EC 2.5.1.6, MetaCyc. August 15th, 2013, from link
-
Ethylene biosynthesis and signaling networks
Wang, K. L.-C.; Li, H.; Ecker J. R. Plant Cell 2002, Supplement, S131-S151