Coding

Part:BBa_K1061002:Design

Designed by: mengyi sun   Group: iGEM13_SYSU-China   (2013-09-11)


RIP 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1437
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1242
    Illegal BsaI.rc site found at 1519
    Illegal SapI.rc site found at 511
    Illegal SapI.rc site found at 1696


Design Notes

It has been reported that besides apoptosis, RIP 1 can also induce necrosis in cells , we also observe this effect in Hep G2 cell lines, and now we are try to find more suitable gene that can only induce apoptosis and avoid the inflammation effect. To use the RIP 1 to treat cancer, tightly control of expression may be necessarily.



Source

We cloning this part from human genomocs via RT-PCR

References