RNA

Part:BBa_K1053120:Design

Designed by: Yasuhito Ito   Group: iGEM13_Tokyo-NoKoGen   (2013-09-10)

RNA scaffold containing HTLV-1 Rex peptide and λN peptide aptamer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 74
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

λN peptide aptamer and Rex peptide aptamer are separated by ten consecutive Thymine sequence. BBa_K1053120 sequence has inserts from the RNA scaffold, have been reportedd by Hung-Wei Yiu and his colleague, to stabilize RNA secondary structure - 45-46 and 83-84

Source

synthesis

References

Hung-Wei Yiu, Vadim V. Demidov, Paul Toran, Charles R. Cantor and Natalia E. Broude, "RNA Detection in Live Bacterial Cells Using Fluorescent Protein Complementation Triggered by Interaction of Two RNA Aptamers with Two RNA-Binding Peptides" Pharmaceuticals 2011, 4, 494-508; doi:10.3390/ph4030494