Coding

Part:BBa_K1053103:Design

Designed by: Eri Takagi   Group: iGEM13_Tokyo-NoKoGen   (2013-09-10)

FA-linker-MS2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The synthesized three-part sequence for FA-linker-MS2 was ligated by overlap PCR.


Source

We used the synthesized sequences of split GFP fused with MS2 and PP7 (FA-linker-MS2, FB-linker-PP7) respectively, reference of BioBrick part BBa_K738004 (FA-linker-MS2) and BBa_K738005 (FB-linker-PP7) (ZJU-China 2012).

References