Generator

Part:BBa_K1053016:Design

Designed by: Ippei Sakamoto   Group: iGEM13_Tokyo-NoKoGen   (2013-09-27)


PBAD-TR(12y)-HHR-taR42-DT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1338
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1145
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1338
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1338
    Illegal AgeI site found at 980
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 962


Design Notes

BBa_K1053012 & BBa_K1053009 Overlap PCR was performed to connect BBa_K1053012 and taR12-DT in BBa_K1053009.


Source

This hammerhead ribozyme(HHR) has trans-acting RNA(taRNA) responsive region, and this HHR responses to taR12y. In absence of taR12y, the HHR can self-cleave, but in presence of taR12y, their capability of self-cleavage is inhibited due to hybridize with taR12y and change their structures. This HHR has taR42 in the downstream. taR12y and taR42 are orthogonal riboregulators reported by Callura et al.


References