Generator
Part:BBa_K1053016:Design
Designed by: Ippei Sakamoto Group: iGEM13_Tokyo-NoKoGen (2013-09-27)
PBAD-TR(12y)-HHR-taR42-DT
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1338
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1145
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1338
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1338
Illegal AgeI site found at 980 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 962
Design Notes
BBa_K1053012 & BBa_K1053009 Overlap PCR was performed to connect BBa_K1053012 and taR12-DT in BBa_K1053009.
Source
This hammerhead ribozyme(HHR) has trans-acting RNA(taRNA) responsive region, and this HHR responses to taR12y. In absence of taR12y, the HHR can self-cleave, but in presence of taR12y, their capability of self-cleavage is inhibited due to hybridize with taR12y and change their structures. This HHR has taR42 in the downstream. taR12y and taR42 are orthogonal riboregulators reported by Callura et al.