Generator

Part:BBa_K1053010:Design

Designed by: Ippei Sakamoto   Group: iGEM13_Tokyo-NoKoGen   (2013-09-27)


PBAD-taR12y-DT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1264
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1145
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1264
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1264
    Illegal AgeI site found at 980
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 962


Design Notes

We had to introduce only two points mutation.


Source

We introduced some mutations to K1053008 by inverse PCR.

References