Generator
Part:BBa_K1053008:Design
Designed by: Ippei Sakamoto Group: iGEM13_Tokyo-NoKoGen (2013-09-27)
PBAD-taR12-DT
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1264
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1145
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1264
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1264
Illegal AgeI site found at 980 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 962
Design Notes
Optimization of transcription start site
Source
Engineered riboregulators enable post-transcriptional control of gene expression Isaacs et al. Nature Biotechnology 22, 841 - 847 (2004)