DNA

Part:BBa_K1051700:Design

Designed by: Yang Zhou   Group: iGEM13_Shenzhen_BGIC_ATCG   (2013-06-29)


SRC1 Intron -4bp at 5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Assembly standard 23

The optimal PCR temperature should be assessed by gradient PCR first in order to maximize the PCR product

Source

From SRC1

References

Nature. 2011 May 25;474(7350):173-8. doi: 10.1038/nature10143. Yeast. 2002 Jan 15;19(1):43-54. J Cell Biol. 2008 Sep 8;182(5):897-910. doi: 10.1083/jcb.200803098. Epub 2008 Sep 1.