Measurement

Part:BBa_K1051288:Design

Designed by: Rui Guan   Group: iGEM13_Shenzhen_BGIC_ATCG   (2013-09-01)


The measurement pathway of ubiquitin fusion protein.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 788
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
    Illegal AgeI site found at 580
    Illegal AgeI site found at 808
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2351


Design Notes

Add the ubiquitin to the N side of GFP.


Source

The promoter is GAL1,with ubiquitin in N side of the GFP, terminator is K1051007.

References