Regulatory

Part:BBa_K105026:Design

Designed by: Katja Karstens   Group: iGEM08_ESBS-Strasbourg   (2008-10-26)


Gal1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This BioBrick ends with a Kozak sequence. To enable the direct fusion with DNA coding domains in frame the vector of this BioBrick has no base pair in between the restriction sites and the BioBrick. <br\n>

For more information about this issus, see:<br\n>

Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).



Source

yeast genomic DNA

References