Tag
hsl1

Part:BBa_K105015:Design

Designed by: Robin Sorg   Group: iGEM08_ESBS-Strasbourg   (2008-10-22)

hsl1 - cell cycle dependent degradation tag in yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 170
    Illegal BglII site found at 185
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To enable the fusion with the protein in frame the vector of this BioBrick has no base pair in between the restriction sites and the BioBrick. Furthermore, this coding sequence does not include a start codon.

For more information about this issus, see:

Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." DSpace http://hdl.handle.net/1721.1/32535 (2006).


Source

Yeast genomic DNA

References

J. L. Burton & M. J. Solomon (2001) Genes Dev 15(18) 2381-2395.