Tag

Part:BBa_K105012:Design

Designed by: Manuel Gersbacher, Katja Karstens   Group: iGEM08_ESBS-Strasbourg   (2008-10-19)

10 aa flexible protein domain linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction sites and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n>

For more information about this issus, see:<br\n>

Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).

Source

Oligos with a yeast optimized coding for the peptide GENLYFQSGG have been ordered. <br\n> The amino acide sequence corresponds to the interdomaine linker used in the reference below.

References

Attila Becskei, Bertrand Séraphin & Luis Serrano (2001) The EMBO Journal 10 2528-2535.