Protein_Domain

Part:BBa_K105002:Design

Designed by: Sandra Dittmann   Group: iGEM08_ESBS-Strasbourg   (2008-10-12)

TUP1 - repressor domain for transcription factors in yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 351
    Illegal NheI site found at 469
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 257


Design Notes

To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction sites and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n>


For more information about this issus, see:<br\n>

Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).

Source

Yeast genomic DNA.

References

Martin Hirst, Cynthia Ho, Luc Sabourin, Michael Rudnicki, Linda Penn & Ivan Sadowski (2001) PNAS 15 8726-8731.