Coding

Part:BBa_K1043009:Design

Designed by: Kevin Thyne   Group: iGEM13_Penn_State   (2013-09-18)


PhaC: Polyhydroxybutyrate synthase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 78
    Illegal BsaI.rc site found at 437
    Illegal BsaI.rc site found at 788


Design Notes

A His tag was added to the C-terminus to avoid folding issues.


Source

Chromobacterium Sp. USM2 amino acid sequence, codon optimized for Physcomitrella patens.

References