Coding

Part:BBa_K1043008:Design

Designed by: Kevin Thyne   Group: iGEM13_Penn_State   (2013-09-18)


NphT7: Acetoacetyl-CoA synthase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 862


Design Notes

The His tag was placed at the N-terminus to avoid issues with protein folding.


Source

Streptomyces amino acid sequence, codon optimized to Physcomitrella patens

References