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Part:BBa_K1040005:Experience

Designed by: Taku Tsuzuki   Group: iGEM13_Osaka   (2013-09-17)


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Applications of BBa_K1040005

  • Ordinarily, E.coli has ilvE gene which encoded a kind of the Ile synthetase.

Under the Ile existence, both E.coli having ilvE and E.coli which did not have it increased. When Ile did not exist, there is a clear difference between the growth rate of E.coli having ilvE and E.coli which did not have it. Also, OD under M63 medium (minimum medium) with Ile differed from it under M63 medium without Ile.

We cultured these two kinds of E.coli in M63 medium with the Ile and without the Ile. And, we measured OD every two hours.

Growth of E.coli(+ilvE,-ilvE strain) without the exsistance of isoleucine
Growth of E.coli(+ilvE,-ilvE strain) in the exsistance of isoleucine


























































  • Experimental results were almost similar to theory, but OD did not increase very much for several hours after start of measurement. Then, it is thought that Ile necessary for E.coli to increase was short. Therefore, starvation response occurred, and it took time to be replaced by the system that mainly synthesized this amino acid.
  • We want to make such a sequence and perform biohazard measures. We add Anhydrotetra cyclin to culture medium. Anhydrotetra cyclin inhibits that TetR binds to ptet, so E.coli with this sequence cannot increase out of culture medium.
experiment and modeling result


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