DNA

Part:BBa_K1039031

Designed by: Urooj Kishor   Group: iGEM13_Waterloo   (2013-09-16)

BXB1 att P

The attB site is the phage attachment site for the phiC31 phage. The site is recognized by phiC31 integrase. The phiC31 integrase catalyzes a unidirectional strand exchange between a 39 bp attP site and a 34 bp attB site (Groth et al., 2000). The enzyme works by making a synapse between attP and attB, making double strand breaks producing a 2 bp sticky overhang, exchanging the strands, and re-ligating them in the recombinant configuration (attL and attR).

Characterization by Fudan_China 2017

  • Group: Fudan_China 2017
  • Author: Haiyun Liu, Fudan University

We characterized the kinetic characterization and the orthogonality between Bxb1 and some other serine recombinase using this attP site. For more information, go to Mycobacterium Phage Bxb1 gp35, DNA integrase

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 27


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