Coding

Part:BBa_K1039023:Design

Designed by: Jama Hagi-Yusuf   Group: iGEM13_Waterloo   (2013-09-13)


BXB1 integrase under lock and key control with lacI expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1660
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
    Illegal BamHI site found at 1934
    Illegal XhoI site found at 2021
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2573
    Illegal NgoMIV site found at 2660
    Illegal AgeI site found at 1710
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2768
    Illegal BsaI.rc site found at 3177


Design Notes

For Bxb1 integrase, the following three silent mutations were made to remove forbidden restriction sites: base 273 G->A base 1269 A->T base 1440 G->A



Source

Some of these parts were synthesized by BioBasic Inc and some were obtained from the iGEM 2013 distribution kit.

References