Composite

Part:BBa_K1036005

Designed by: Zhaopeng Cheng, Xiyu Wu   Group: iGEM13_XMU-China   (2013-09-09)

sfgfp (with LVA-tag) under plux R control

Description

This part consists of GFP variant Superfolder GFP with a LAV-tag and a quorum sensing promoter controlled luxI protein gene. Superfolder GFP with a LAV-tag is introduced in Part:BBa_K1036004, and quorum sensing mechanism is described in Part:BBa_K1036003.

Experimental Data

Fig.1 showed the correct result of this part construction and Fig.2 showed the distinguish of BBa_K1036005 which was without lux promoter and BBa_K1036006 which was under lux promoter control.






Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2737
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 914
    Illegal BsaI.rc site found at 2017
    Illegal SapI.rc site found at 31


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Categories
Parameters
None