Coding
Part:BBa_K1033260:Design
Designed by: Anders Edlund Group: iGEM13_Uppsala (2013-09-13)
Toxin-antitoxin system from Lactobacillus plantarum
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 158
Illegal SpeI site found at 534 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 158
Illegal SpeI site found at 534 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 158
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 158
Illegal SpeI site found at 534 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 158
Illegal SpeI site found at 534 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part still contains two illegal restriction sites for EcorI and SpeI. However since it doesn't matter where in your construct you put the system it can still be assembled with XbaI and PstI.
Source
The Lactobacillus plantarum NC7 plasmid p256.
References
Plasmid p256 from Lactobacillus plantarum represents a new type of replicon in lactic acid bacteria, and contains a toxin–antitoxin-like plasmid maintenance system, Microbiology, Elisabeth Sorvig et al. September 30 2004