Plasmid_Backbone

Part:BBa_K1033201:Design

Designed by: Viktor Blomkvist   Group: iGEM13_Uppsala   (2013-08-23)


pSBLb4E15 E. coli and lactobacilli shuttle vector with erythromycin resistance


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

Had to make sure it was compatible with biobrick standard 10


Source

Ori in pEL4C15 replaced with ori from pJP059. Chloramphenicol resistance replaced with erythromycin resistance.

References