Composite
Part:BBa_K1030000:Design
Designed by: Wei Huang Group: iGEM13_SUSTC-Shenzhen-A (2013-09-16)
J23100->tetA-asv+sfGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1106 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 960
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 406
Illegal NgoMIV site found at 566
Illegal NgoMIV site found at 934 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1273
Design Notes
We tuned the expression level of tetA using a series of synthetic constitutive promoters. Different levels of tetA will make E coli vulnerable at different level of extracellular Nickel ion, as it is transported into cell via tetA.
Source
J23100 is an synthetic constitutive promoter from iGEM tetA is an tetracycline transporter from pBR322 sfGFP is superfolder GFP, from iGEM