Composite

Part:BBa_K1030000:Design

Designed by: Wei Huang   Group: iGEM13_SUSTC-Shenzhen-A   (2013-09-16)


J23100->tetA-asv+sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1106
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 960
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 406
    Illegal NgoMIV site found at 566
    Illegal NgoMIV site found at 934
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1273


Design Notes

We tuned the expression level of tetA using a series of synthetic constitutive promoters. Different levels of tetA will make E coli vulnerable at different level of extracellular Nickel ion, as it is transported into cell via tetA.


Source

J23100 is an synthetic constitutive promoter from iGEM tetA is an tetracycline transporter from pBR322 sfGFP is superfolder GFP, from iGEM

References