Regulatory

Part:BBa_K102911

Designed by: Wayne Materi   Group: iGEM08_Alberta_NINT   (2008-10-26)

TA13 gate from synthetic algorithm v1.2

This design incorporate stem bulges and YUNR sequence in the loop (5' UUGG) as per Isaacs et al, 2004 to improve RNA stability and susceptibility to anti-sense induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 14
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Data sheet

NINT Mfold-K102911.jpg


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Categories
Parameters
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