Other

Part:BBa_K1028003:Design

Designed by: Joseph Beton   Group: iGEM13_Leeds   (2013-09-24)

Silica Detection System


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 62
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 472
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1917


Design Notes

The biobricks were digested and ligated together using the RFC10 restriction sites

This part uses a strong RBS to both maximize production of the INP.Si4 complex and the fluorescence response of GFP production from activation of pCpxR


Source

The DNA for this part was taken from the registry

References