Coding

Part:BBa_K1027001:Design

Designed by: Robert Harrison, Lorna Hepworth, Vun Hiang Tan   Group: iGEM13_Manchester   (2013-09-19)


Delta 9 Desaturase, optimised for expression in Escherichia coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 447
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 271
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for expression in E. coli


Source

Synthesized gene based off the protein sequence from Synechocystis sp

References