Coding

Part:BBa_K1026003:Design

Designed by: Hongyi WU   Group: iGEM13_SJTU-BioX-Shanghai   (2013-09-16)


gRNA targeting mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Criteria for designing a gRNA in CRISPRi are illustrated in the above literature.



Source

The gRNA sequence is de novo synthesized.

References