Composite

Part:BBa_K1025009:Design

Designed by: Weifan Liang   Group: iGEM13_Tsinghua-E   (2013-09-15)


Sweet Pressure Part （RBS38）


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2705
    Illegal XbaI site found at 2732
    Illegal PstI site found at 2680
    Illegal PstI site found at 2744
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2705
    Illegal PstI site found at 2680
    Illegal PstI site found at 2744
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2705
    Illegal BglII site found at 2102
    Illegal BamHI site found at 236
    Illegal BamHI site found at 2726
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2705
    Illegal XbaI site found at 2732
    Illegal PstI site found at 2680
    Illegal PstI site found at 2744
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2705
    Illegal XbaI site found at 2732
    Illegal PstI site found at 2680
    Illegal PstI site found at 2744
    Illegal AgeI site found at 71
    Illegal AgeI site found at 2117
    Illegal AgeI site found at 2549
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2928
    Illegal SapI site found at 53


Design Notes

The genes were cloned easily by PCR and connected to plasmid backbone by in-fusion cloning.


Source

tnaC and Rho were sythesized by DNA 2.0 corporation according to reference[1].MalQ was sythesized by DNA 2.0 corporation with codon optimization according to GenBank DQ019991. [1]Gong, F. & Yanofsky, C. Instruction of translating ribosome by nascent peptide. Science297, 1864-1867, doi:10.1126/science.1073997 (2002).


References