Composite

Part:BBa_K1025008:Design

Designed by: Weifan Liang   Group: iGEM13_Tsinghua-E   (2013-09-15)


Sweet Pressure Part （RBS34）


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2708
    Illegal XbaI site found at 2735
    Illegal PstI site found at 2683
    Illegal PstI site found at 2747
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2708
    Illegal PstI site found at 2683
    Illegal PstI site found at 2747
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2708
    Illegal BglII site found at 2105
    Illegal BamHI site found at 236
    Illegal BamHI site found at 2729
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2708
    Illegal XbaI site found at 2735
    Illegal PstI site found at 2683
    Illegal PstI site found at 2747
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2708
    Illegal XbaI site found at 2735
    Illegal PstI site found at 2683
    Illegal PstI site found at 2747
    Illegal AgeI site found at 71
    Illegal AgeI site found at 2120
    Illegal AgeI site found at 2552
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2931
    Illegal SapI site found at 53


Design Notes

The genes were cloned easily by PCR and connected to plasmid backbone by in-fusion cloning.


Source

tnaC and Rho were sythesized by DNA 2.0 corporation according to reference[1].MalQ was sythesized by DNA 2.0 corporation with codon optimization according to GenBank DQ019991. [1]Gong, F. & Yanofsky, C. Instruction of translating ribosome by nascent peptide. Science297, 1864-1867, doi:10.1126/science.1073997 (2002).


References