Composite

Part:BBa_K1025007:Design

Designed by: Weifan Liang   Group: iGEM13_Tsinghua-E   (2013-09-15)


Bitter Defender Part（RBS_B0032）


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 616
    Illegal NgoMIV site found at 691
    Illegal NgoMIV site found at 721
    Illegal NgoMIV site found at 941
    Illegal NgoMIV site found at 1059
    Illegal NgoMIV site found at 1326
    Illegal NgoMIV site found at 1521
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1748


Design Notes

The genes were cloned easily by PCR and connected to plasmid backbone by in-fusion cloning.


Source

TnaC and Rho were sythesized by DNA 2.0 corporation according to reference[1]. RBS_B0032 was from the biobrick B0032. TetA was cloned from plasmid pCM110. [1]Gong, F. & Yanofsky, C. Instruction of translating ribosome by nascent peptide. Science297, 1864-1867, doi:10.1126/science.1073997 (2002).


References