Composite

Part:BBa_K1025005:Design

Designed by: Weifan Liang   Group: iGEM13_Tsinghua-E   (2013-09-14)


Tryptophan-sensor Part


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3394
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3394
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3394
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3394
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3394
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3623


Design Notes

The genes were cloned easily by PCR and connected to plasmid backbone by in-fusion cloning.


Source

TanC and Rho were synthesized by DNA 2.0 corporation according to reference[1]. LacZ was cloned from E.coli genome. [1]Gong, F. & Yanofsky, C. Instruction of translating ribosome by nascent peptide. Science297, 1864-1867, doi:10.1126/science.1073997 (2002).

References