Composite

Part:BBa_K1022125

Designed by: Maithili Krishnan   Group: iGEM13_TU-Delft   (2013-09-20)

pT7: RBS: His6- SUMO: Signiferin: RBS: TetR:TT : pTet: cI: TT : pcI: Ulp : Lysis

This biobrick is the one of the three final constructs of the project Peptidor by Team TU Delft iGEM 2013. This has a Promoter pT7 along with a His6 SUMO tagged peptide followed by a TetR which suppresses the timer module containing promoter pTET, cI is produced which suppresses pcI. Then Ulp protease is produced following by a Kill switch lysis device.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 46
    Illegal NheI site found at 2346
    Illegal NheI site found at 4441
    Illegal NheI site found at 4464
    Illegal NotI site found at 3042
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 170
    Illegal BamHI site found at 3011
    Illegal XhoI site found at 3020
    Illegal XhoI site found at 3051
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 355
    Illegal AgeI site found at 4046
    Illegal AgeI site found at 4116
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 4697

Information

This part is one of the final constructs designed in the project [http://2013.igem.org/Team:TU-Delft/Project Peptidor] by Team TU Delft

Please visit [http://2013.igem.org/Team:TU-Delft iGEM 2013 Team TU Delft] for more


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