Generator

Part:BBa_K1021029:Design

Designed by: Swati Sureka   Group: iGEM13_Cornell   (2013-09-13)


PtrpC+nptII+TtrpC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1171
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1004
    Illegal NgoMIV site found at 1669
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1385
    Illegal SapI.rc site found at 853
    Illegal SapI.rc site found at 1063


Design Notes

The terminator is not strictly necessary in fungi, but was added to improve BBa_K1021015.


Source

PtrpC and TtrpC, both from A. nidulans, came from the pUCATPH transformation vector. The nptII gene from E. coli K12 was taken from the pNG transformation vector.

References