Generator

Part:BBa_K1021027:Design

Designed by: Swati Sureka   Group: iGEM13_Cornell   (2013-09-13)


PgpdA + nptII


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1525
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1358
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1207
    Illegal SapI.rc site found at 1417


Design Notes

The construct does not contain an RBS, as it was designed for fungal expression; refer to the deisgn of the basic subparts for more details.


Source

PgpdA came from pBARGPE1 from the FGSC (originally Aspergillus nidulans) and nptII originated from the pNG transformation vector (originally E. coli K12).

References