Part:BBa_K1021020:Experience

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Applications of BBa_K1021020

This construct was modified to contain a bacterial RBS so that we could characterize its transcriptional functionality. A fluorescence assay was run using a 96 well plate as described in the protocol section. T7+RBS+GFP and T7+GFP (BBa_K1021020) constructs in E. coli BL21-A1 were examined for their relative fluorescence (fluorescence/OD600). A T7 construct in E. coli BL21-A1 without downstream elements was utilized as a control along with the use of the aforementioned constructs in E. coli DH5α. As can be seen from the graph there were evident differences in peak fluorescence between the T7+RBS+GFP construct and the T7+GFP construct and controls. The T7+GFP+RBS construct appears to have a 20 fold higher relative fluorescence than the T7+GFP construct or the controls.

User Reviews

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