Part:BBa_K1021019
PT7+crtY
The crtY gene (the fourth component of the carotenoid biosynthesis pathway) can be used to convert lycopene β-carotene in the presence of T7 RNA polymerase. It was designed for use in fungi.
Adapted from KEGG reference pathways
crtY(BBa_K539119) was originally from the 2011 NCTU Taiwan team that isolated the coding sequence of crtY from Pantoea ananatis (Accession number D90087). crtY encodes lycopene β-cyclase, part of the carotenoid biosynthesis pathway, which converts lycopene to β-carotene (Misawa et al., 1990).
The T7 promoter part was originally from the 2007 Ljubljana team (BBa_I712074). It was used in order to assemble multiple genes into a single vector. Fungal promoters are quite long and therefore difficult to assemble, and E. coli will often recombine over and remove direct repeats within DNA sequences. Because the T7 promoter is a short sequence, each of the carotenoid genes could be put behind the T7 promoter and assembled together for maximum simultaneous transcription of the carotenoid genes.
1. Misawa, N., Nakagawa, N., Kobayashi, K., Yamano, S., Nakamura, K., and Harashima, K. 1990. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. Journal of Bacteriology 172, 6704-6712.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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