Part:BBa_K1021018
PT7+crtI
The crtI gene (the third component of the carotenoid biosynthesis pathway) sits downstream of the T7 promoter. It was designed for use in fungi.
Adapted from KEGG reference pathways
crtI (BBa_K118003) was originally from the 2008 Edinburgh team that isolated it from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene dehydrogenase, part of the carotenoid biosynthesis pathway, which converts phytoene to lycopene (Misawa, et al., 1990).
The T7 promoter part was originally from the 2007 Ljubljana team (BBa_I712074). It was used in order to assemble multiple genes into a single vector. Fungal promoters are quite long and therefore difficult to assemble, and E. coli will often recombine over and remove direct repeats within DNA sequences. Because the T7 promoter is a short sequence, each of the carotenoid genes could be put behind the T7 promoter and assembled together for maximum simultaneous transcription of the carotenoid genes.
1. Misawa, N., Nakagawa, N., Kobayashi, K., Yamano, S., Nakamura, K., and Harashima, K. 1990. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. Journal of Bacteriology 172, 6704-612. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 123
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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