Coding
Part:BBa_K1021003:Design
Designed by: Swati Sureka Group: iGEM13_Cornell (2013-08-30)
bar (bialaphos resistance)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 545
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 54
Illegal NgoMIV site found at 205
Illegal NgoMIV site found at 451 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The plasmid sequence was unknown so extensive sequencing was carried out in order to extract it from its original vector, confirm BioBrick compatibility, and transfer it to pSB1C3.
Source
pBARGPE1 from the FGSC (http://www.fgsc.net/fgn/pall.html)