Part:BBa_K1020005:Design

Alkane Marker--Device for detection of various alkanes

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1028
  Illegal NheI site found at 1051
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 752
  Illegal AgeI site found at 864
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1821
  

Design Notes

We chooce BBa_J23103 as the promoter of AlkR gene, because we assume that overexpress AklR protein will have a negative influence on cell's growth.

Source

Acinetobacter baylyi ADP1