Coding

Part:BBa_K1014001:Design

Designed by: Xiangmiao Zeng   Group: iGEM13_HIT-Harbin   (2013-08-27)

HrpR Gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 534
    Illegal AgeI site found at 121
    Illegal AgeI site found at 127
    Illegal AgeI site found at 175
    Illegal AgeI site found at 741
    Illegal AgeI site found at 766
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TAATAA double stop codon added.

Codon optimization for E.coli was done using [http://www.jcat.de/ JCat] an online Codon Adaptation Tool which was published in NAR (Nucleic Acids Research).

Codon optimization results was present on [http://2013.igem.org/Team:HIT-Harbin/Parts our wiki]

Source

[http://www.ncbi.nlm.nih.gov/nuccore/NC_007005.1?report=genbank&from=1351297&to=1352241 GeneBank NC_007005.1]

BBa_M39015

References

[http://www.pnas.org/content/102/31/11064.short Proc. Natl. Acad. Sci. U.S.A. 102 (31), 11064-11069 (2005)]

[http://nar.oxfordjournals.org/content/33/suppl_2/W526.short Andreas Grote et al. JCat: a novel tool to adapt codon usage of a target gene to its potential expression host]